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KMID : 1004820120130010035
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Journal of Biomedical Research
2012 Volume.13 No. 1 p.35 ~ p.46
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Morphological and Histological Changes in Photoaged Hairless Mice Induced by Phellodendrin cortex Water Extract Application
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Bae In-Soon
Shim Mi-Ja
Park Mi-Soon
Kim Young-Chul
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Abstract
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This study was conducted in order to evaluate the alleviating effects of Phellodendrin cortex water extract (PCWE) on skin aging in hairless mice via observation of morphogical and histological changes. Skin aging was induced by UVB irradiation and application of squalene monohydroperoxide (Sq-OOH) to the back skin of hairless mice for six weeks. And, at the same time, saline (C), jojoba oil (VC), PCWE (E), and 0.01% retinoic acid diluted with polyethylene glycol (PC) were applied topically twice per day, six days per week, for a period of six weeks. Improved wrinkle formation in a pattern of shallow furrows and thin and narrow crests was observed in the retinoic acid and PCWE application groups, compared to the C group. On the morphologic analysis for skin wrinkles, the E group showed lower levels in skin roughness, maximum roughness, average roughness, smoothness depth, and arithmetic average roughness by 13.1, 17.2, 18.4, 15.4, and 16.1%, respectively, compared with the C group, indicating that PCWE inhibited potential formation of wrinkles in the skin. In the C group, structures of lipid lamellae and collagen fibers were broken or deformed with an irregular arrangement. Application of retinoic acid and PCWE protected against the deformity of lipid lamellae and collagen fibers. Elastic fibers in dermis of the C group also showed severe transformation; however, applications of retinoic acid and PCWE resulted in a significant decrease in the number of denatured elastic fibers. Therefore, PCWE could have an alleviating effect on skin aging induced by UVB irradiation and application of Sq-OOH.
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KEYWORD
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Phellodendrin cortex water extract, Skin aging, Collagen fiber, Elastic fiber
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